Swine Tests and Fees

Acid Fast Stain

Tissues, aspirates, exudates, body fluids, feces in a sterile leakproof container; shipped chilled overnight. Impression smears (skin lesions) on slide within a slide folder.

1 day

$7

ACTH (Baseline)

1 ml of plasma from a purple top tube (EDTA) from all species (no primates or avian); shipped frozen overnight from Monday to Thursday. Collect blood and place it in an EDTA blood tube. Centrifuge within 1 hour of collection, remove the plasma and place 1 ml in an additive free plastic tube. Place the tube in a secondary container with water, freeze the sample in the container filled with water. Ship frozen overnight from Monday to Thursday.

1 day

$30

Aerobic Culture

Fresh tissues or body fluids in a sterile leakproof container or syringe; shipped chilled within 24 hours of collection. Swabs in aerobic transport media; shipped chilled overnight within 24 hours of collection.

3-5 days

$10

African Swine Fever (ASF) Virus qPCR

Whole blood, tonsil, spleen or lymph node from a porcine in a leakproof and sterile tube or container; shipped chilled overnight

1-3 days

This is an exotic disease. Request the FAD number from State Veterinarian before sample shipment if submission is for NAHLN FAD investigation.

$45 No Charge for NAHLN FAD Investigation

Anaerobic Culture

Fresh tissues or body fluids in a sterile leakproof container or syringe; shipped chilled overnight within 24 hours of collection. Swabs in anaerobic transport media; shipped chilled overnight within 24 hours of collection.

3-5 days

$12

Antimicrobial Susceptibility Panels

Pure cultures of isolated pathogen or as requested by the submitter.

1-3 days after ID

Up to 3 isolates per specimen.

$10 per isolate

Bacterial Identification by MALDI-TOF

Culture plates for bacterial ID; shipped chilled within 24 hours of collection.

1-2 days

$10

Baermann Test (Lungworm)

10 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$8

Biopsy

Fixed tissues in a leakproof container with a screw-on lid (should be put in a Ziploc bag with paper towels/absorbent material).

5 days

$40.00 first tissue specimen per animal $10.00 each subsequent tissue specimen

Brain Removal and Large Complex Fresh Tissue Handling

Head or fresh carcass (preferred not frozen), double bagged inside a leakproof Styrofoam or plastic cooler; shipped chilled overnight. For rabies testing, contact your county Department of Health (DOH) prior to submission for approval.

1 day

$30 per animal

Brucella abortus/suis Serum Test (BAPA, Card, Rivonal, RAP)

1 ml of serum from a bovine, camelid, caprine, cervid, ovine, porcine or exotic in a serum separator tube (red top/tiger top); shipped chilled overnight.

1-3 days

$5

Campylobacter Culture

Aborted fetus or fetal lung, liver, stomach content, placenta, semen, cervical or vaginal mucus in Campylobacter transport medium; shipped chilled overnight or delivered as soon as possible. Feces in a leakproof container or fecal swab in Cary-Blair or another suitable transport media; shipped chilled overnight or delivered as soon as possible.

5-7 days

Fresh or fixed large tissues, viscera, or aborted fetuses submitted to the lab for any test may be charged the Large/Complex Dissection fee if additional handling is required prior to performing the requested test.

$12

CBC Other Mammals

2 ml of blood from a canine, feline, guinea pig, rat, or rabbit in a purple top tube (EDTA); shipped chilled overnight.

1 day

$15

Chemistry — Large Animal Profile

2 ml of serum from a bovine, ovine, caprine, porcine, alpaca, or llama in a red top tube; shipped chilled overnight. Includes: Albumin (ALB), Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), Calcium (Ca), Creatine Kinase (CK), Total Protein (TP), Gamma Glutamyl Transferase (GGT), Globulin (GLOB), Magnesium (Mg), Phosphorus (PHOS), Blood Urea Nitrogen (BUN).

1 day

$20

Classical Swine Fever qPCR

Tonsil, tonsil scraping, spleen or lymph node from a porcine in a leakproof and sterile tube or container; shipped chilled overnight.

1-3 days

This is an exotic disease. Request the FAD number from State Veterinarian before sample shipment if submission is for NAHLN FAD investigation.

$45 No Charge for NAHLN FAD Investigationn

Cortisol

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours. Separate serum from clot and ship overnight with ice packs.

1 day

$25

Cryptosporidium Fecal Direct Fluorescent Antigen Test (DFA)

2 gm of fresh feces from all animals in a leakproof container; shipped chilled overnight.

1 day

$20

Cytology

Air-dried or fixed slides submitted in a plastic or cardboard slide container or leakproof tube for fluid samples. Fluids in a leakproof tube (preferably preserved in EDTA). Unpreserved fluid should be shipped chilled, overnight. Do not ship for Saturday delivery. Culture swabs are unsuitable for cytology.

2 days

$30 per site

Electrolyte Plus Panel

1 ml of heparinized whole blood (green top) or serum (red top) from a canine, feline, equine, bovine, caprine, ovine; shipped chilled overnight. Included parameters: Chloride, Potassium, Sodium, tC02

1 day

$15

Estradiol

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours. Separate serum from clot and ship overnight with ice packs.

1 day

$30

Fecal Centrifugation Sucrose

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Culture (Aerobic & Salmonella)

Feces in a leakproof container; shipped chilled overnight.

2-5 days (serotyping additional 5-10 days)

$20

Fecal Examination  — Direct Smear

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Examination  — Zinc Sulfate

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Floatation — Zinc Sulfate

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Occult Blood

2 gm of feces in a sealed leakproof container; shipped chilled overnight.

1 day

$10

Fecal Sedimentation

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$8

Fibrinogen

1 ml of blood from a canine or feline in a blue top tube (Sodium citrate); shipped chilled overnight. 1 ml of blood in a purple top tube (EDTA ) from all other species; shipped chilled overnight.

1 day

$15

Foot and Mouth Disease (FMD) Virus qPCR

Vesicular tissue or fluid, crusts, scabs, oral or lesions swabs from a bovine, porcine, caprine, ovine, camelid or cervid (cloven-hoofed zoo animal or wildlife) in a sterile leakproof tube or container; shipped chilled overnight.

1-3 days

This is an exotic disease. Request the FAD number from State Veterinarian before sample shipment if submission is for NAHLN FAD investigation.

$30

Fungal Culture

Swabs, fresh tissue, or skin scrapings in a sterile container; shipped chilled overnight. Not for environmental samples.

7-28 days

$15

Fungal Identification

Plate or dermatophyte media; shipped chilled overnight. Not for environmental samples.

7-28 days

$10

Giardia Fecal Direct Fluorescent Antigen Test (DFA)

2 gm of fresh feces from all animals in a leakproof container; shipped chilled overnight.

1 day

$20

Gram Stain

Tissues, aspirates, exudates in a sterile leakproof container; shipped chilled overnight. Impression smears on slide within a slide folder.

1 day

$5

Influenza A qPCR Matrix (Mammalian)

Respiratory or nasal swabs in 3 ml of viral transport medium (VTM) or fresh lung; shipped chilled overnight. Samples accepted from feline, equine, porcine, canine and pinnipeds. Do not pool swabs. Swabs must be received within 4 working days of collection. Use synthetic swabs with a plastic handle. Avoid cotton or calcium alginate swabs or swabs with wooden handles.

1-3 days

Tests run Tuesday and Friday.

$30

Insulin

1 ml of serum in a red top tube from all species (no primates or avian); shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$25

Large/Complex Dissection

Fresh or fixed large tissues or viscera, double bagged inside a leakproof container; shipped chilled overnight if fresh tissues.

1-2 days

$30

Leptospira spp. qPCR

Urine: minimum of 5 mL for small animals or 10 mL for large animals in leakproof and sterile container; shipped chilled overnight. Tissue: kidney, liver, placenta in leakproof and sterile container; shipped chilled overnight. Whole blood in EDTA (purple top) tube; shipped chilled overnight. Animal species: multiple species

2-4 days

Tests run on Tuesday and Friday.

$30

Leptospirosis IHC

Kidney in formalin in a leakproof container.

10 days

$25

Leptospirosis Microscopic Agglutination Test 5 (Tests for 5 serovars: L. Pomona; L. ictero; L. canicola; L. grippo and L. hardjo)

1 ml of serum in a serum separator tube (red top/tiger top); shipped chilled overnight.

3-5 days

Tests run on Wednesday. Samples received prior to 10:30 AM on Wednesday will be tested same day.

$15

McMaster’s Egg Count

1 gm minimum of fresh feces in a leakproof container; shipped chilled overnight.

1-2 days

$10

Methicillin-resistant Staphylococcus confirmation (mecA qPCR)

Pure Staphylococcus aureus and/or Staphylococcus pseudintermedius isolates; shipped chilled overnight.

1-3 days

Tests run Monday and Thursday.

$30

Mycobacterium Culture

Tissue or aspirate, body fluids, or feces in a sterile leakproof container or sealed bag; shipped chilled overnight. Specimens must be submitted as soon as possible, or at most within 24 hours of collection.

56 days

If positive for Mycobacterium growth, sent to FL-DOH or NVSL for identification.

$20

Mycoplasma Culture — Food & Fiber Animal

Fresh tissue from cattle, swine, goats, sheep, poultry, captive cervids, alpacas, llamas, or rabbits in a sterile leakproof container or swabs in Mycoplasma transport medium; shipped chilled overnight and received within 24 hours of collection.

7-10 days

$10

Necropsy Exam — Food and Fiber Animal

Fresh carcass of cattle, swine, goats, sheep, poultry, rabbits, captive cervids, llamas, or alpacas (preferred not frozen), double bagged inside a leakproof container or cooler; shipped chilled overnight.

20 days depending on ancillary testing

$100 per animal

Necropsy Exam — Forensics, Research, and Insurance

Fresh carcass (preferred not frozen), double bagged inside a leakproof Styrofoam or plastic cooler; shipped chilled overnight.

20 days depending on ancillary testing

$250 per animal

Necropsy Exam — Other Animal

Fresh carcass of an equine, canine, feline, rodent, or reptile (preferred not frozen) double bagged inside a leakproof cooler; shipped chilled overnight.

20 days depending on ancillary testing

$150 per animal plus cremation fee

Necropsy in a Bottle (Microscopic Examination)

Fixed tissues from a necropsy not performed at the BADDL shipped in a leakproof container.

10 days

$75 per animal

Parasite Identification

Larvae or other parasite in 70% alcohol in a sealed leakproof container.

5-10 days

$15

Phenobarbital

1 ml of serum in a red top tube from all species (no primates or avian); shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$30

Progesterone

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a non-additive clot tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples. Usually within the next 4 hours to avoid loss of progesterone.

1 day

$20

Pseudorabies g1 ELISA

1 ml of serum from swine in a serum separator tube (red top/tiger top) or other sterile tube; shipped chilled overnight. The PRV g1 ELISA is the official differential test for use in the Cooperative State/Federal PRV eradication program. All PRV samples will be screened with the PRV gB ELISA unless the pig or herd is known to be vaccinated with a g1-deleted vaccine and is being screen for exposure to the wild-type virus.

3-5 days

2-3 days Tests run on Tuesday for samples received prior to 12:00 PM. Tests run on Friday for samples received by 5:00 PM the day prior.

$10 No Charge for Surveillance

Pseudorabies gB ELISA

1 ml of serum from swine in a serum separator tube (red top/tiger top) or other sterile tube; shipped chilled overnight.

2-3 days

2-3 days Tests run on Monday and Thursday for samples received prior to 12:00 PM

$10 No Charge for Surveillance

Rabies (Non-Exposure)

Head or carcass (not frozen), double bagged inside a leakproof container; shipped chilled overnight. Requires the examination of a complete cross-section of the brain stem and cerebellum (including vermis, right & left lobes). If the cerebellum is not available, a diagnosis can be made by the examination of the brain stem and hippocampi (right and left).

1-3 days

$40

RPLN/Obex Collection

Head or fresh carcass (preferred not frozen), double bagged inside a leakproof container or cooler; shipped chilled overnight.

1-2 days

$30 per animal

Salmonella Culture (Grouping and/or Serotyping on Request)

Chick box papers, drag swabs, boot swabs, chick meconium, and fluff, feces, tissues, or body fluids in a sterile, leakproof container; shipped chilled overnight.

2-5 days (serotyping additional 5-10 days)

$15

Salmonella Culture - Serogrouping

Chick box papers, drag swabs, boot swabs, chick meconium, and fluff, feces, tissues, or body fluids in a sterile, leakproof container; shipped chilled overnight.

1-2 days

$5

Slide/Block Preparation

Fixed tissue submitted in formalin in a leakproof container. Duplicate slides can be made for send-out.

3-4 days

$15 each

Spinal Cord Removal

Fresh carcass (preferred not frozen), double bagged inside a leakproof Styrofoam or plastic cooler; shipped chilled overnight.

20-25 days

$50 per animal

Testosterone

1 ml of serum in a red top tube from all species (no primates or avian); shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$20

Thyroid Panels – TSH+TP1 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$30 (includes TSH + 1 choice in panel)

Thyroid Panels – TSH+TP2 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$35 (includes TSH + 2 choices in panel)

Thyroid Panels – TSH+TP4 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$55 (includes TSH + 4 choices in panel)

Thyroid Panels — TSH+TP2 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$35 (includes TSH + 2 choices in panel)

Tick Identification

Tick in 70% alcohol in a sealed leakproof container.

5-10 days

Confirmation of exotic or non-endemic ticks may take up to one month at NVSL.

$15

Urine Analysis (Cytology & Fluid Chemistry Analysis)

10-15 ml urine in a sealed leakproof tube; shipped chilled overnight

1 day

$35

Vesicular Stomatitis (VS) Virus Neutralization (Indiana Strain)

1 ml of serum from a bovine, equine, porcine, caprine or ovine in a sterile container; shipped chilled overnight.

4-7 days

Tests run Monday, Wednesday and Friday for samples received the day prior. Suspect samples forwarded to NVSL for additional analysis and confirmation. * Unless specified on the submission form, samples submitted will have both Indiana and New Jersey strains VN performed.

$12 per strain

Vesicular Stomatitis (VS) Virus Neutralization (New Jersey Strain)

1 ml of serum from a bovine, equine, porcine, caprine or ovine in a sterile container; shipped chilled overnight.

4-7 days

Tests run Monday, Wednesday and Friday for samples received the day prior. Suspect samples forwarded to NVSL for additional analysis and confirmation. * Unless specified on the submission form, samples submitted will have both Indiana and New Jersey strains VN performed.

$12 per strain

Virus Isolation

Fresh tissues, swabs, scrapings, body fluids, secretions, urine, blood or whole blood from a non-primate animal submitted in a sterile container.

14-28 days

If required, the suspected cell cultures will be sent to an outside lab for ID confirmation.

$30

Virus Isolation — Enhanced — Food and Fiber Animals

Fresh tissues, swabs, scrapings, body fluids, secretions, urine, blood or whole blood from a non-primate animal submitted in a sterile container.

14-28 days

If required, the suspected cell cultures will be sent to an outside lab for ID confirmation.

$30