Poultry/Non-Poultry Avian Tests and Fees

Acid Fast Stain

Tissues, aspirates, exudates, body fluids, feces in a sterile leakproof container; shipped chilled overnight. Impression smears (skin lesions) on slide within a slide folder.

1 day

$7

Aerobic Culture

Fresh tissues or body fluids in a sterile leakproof container or syringe; shipped chilled within 24 hours of collection. Swabs in aerobic transport media; shipped chilled overnight within 24 hours of collection.

3-5 days

$10

Anaerobic Culture

Fresh tissues or body fluids in a sterile leakproof container or syringe; shipped chilled overnight within 24 hours of collection. Swabs in anaerobic transport media; shipped chilled overnight within 24 hours of collection.

3-5 days

$12

Antimicrobial Susceptibility Panels

Pure cultures of isolated pathogen or as requested by the submitter.

1-3 days after ID

Up to 3 isolates per specimen.

$10 per isolate

Avian Influenza Agar Gel Immunodiffusion

1 ml of serum from an avian in a serum separator tube (red top/tiger top) or a bullet tube; shipped chilled overnight.

2-4 days

Tests run on Tuesday and Thursday.

$5 No charge for surveillance

Avian Influenza Virus qPCR (matrix)

Poultry - Tracheal or oropharyngeal swabs in 3 ml of viral transport medium (VTM) for pools of up to 5 birds or 5.5 ml of VTM for pools of up to 11 birds; shipped chilled overnight. Captive Pet Birds - Cloacal swabs in 3 ml of VTM broth for pools of up to 5 birds by species; shipped chilled overnight. Wild/Free Roaming Waterfowl – 1 Tracheal and 1 Cloacal from the same bird in 3 ml VTM; shipped chilled overnight. Do not pool more than one bird. Poultry environmental swabs in 5.5 ml of VTM broth for pools of up to 5 swabs. Fresh respiratory, enteric, reproductive, or brain tissue (if neurologic) in a leakproof and sterile container; shipped chilled overnight. Swabs must be received within 4 working days of collection. Use synthetic swabs with a plastic handle. Avoid cotton or calcium alginate swabs or swabs with wooden handles.

1-3 days

Tests run Tuesday and Friday.

$30 each subtype

Avian Influenza Virus qPCR (poultry H5 or H7 subtyping)

Suspect tracheal, cloacal or oropharyngeal swabs, poultry environmental swabs, or fresh or frozen tissues.

1-3 days

Tests run Tuesday and Friday.

$10 per isolate

Avian Influenza  ELISA

1 ml of serum from a chicken or turkey in a serum separator tube (red top/tiger top) or other sterile tube; shipped chilled overnight.

2-3 days

Tests run on Thursday for samples received prior to 12:00 PM.

$2 No charge for surveillance

Bacterial Identification by MALDI-TOF

Culture plates for bacterial ID; shipped chilled within 24 hours of collection.

1-2 days

$10

Biopsy

Fixed tissues in a leakproof container with a screw-on lid (should be put in a Ziploc bag with paper towels/absorbent material).

5 days

$40.00 first tissue specimen per animal $10.00 each subsequent tissue specimen

Brain Removal and Large Complex Fresh Tissue Handling

Head or fresh carcass (preferred not frozen), double bagged inside a leakproof Styrofoam or plastic cooler; shipped chilled overnight.

1-2 days

$30 per animal

Campylobacter Culture

Aborted fetus or fetal lung, liver, stomach content, placenta, semen, cervical or vaginal mucus in Campylobacter transport medium; shipped chilled overnight or delivered as soon as possible. Feces in a leakproof container or fecal swab in Cary-Blair or another suitable transport media; shipped chilled overnight or delivered as soon as possible..

5-7 days

Fresh or fixed large tissues, viscera, or aborted fetuses submitted to the lab for any test may be charged the Large/Complex Dissection fee if additional handling is required prior to performing the requested test.

$12

Chemistry — Comprehensive Diagnostic Profile

2 ml of serum from all other species (No primates) in a red top tube; shipped chilled overnight. Includes: Alanine Aminotransferase (ALT), Albumin (ALB), Alkaline Phosphatase (ALP), Amylase (AMY), Calcium (Ca), Creatinine (CRE), Glucose (GLU), Globulin (GLOB), Phosphorus (PHOS), Potassium (K+), Sodium (Na+), Total Bilirubin (TBIL), Total Protein (TP), Blood Urea Nitrogen (BUN).

1 day

$30

Chlamydia spp. qPCR

Cloacal, choanal or throat swabs from live birds. 1 gm of feces from birds in a leakproof and sterile container. 1 gm of fresh tissue (liver, spleen, lung or kidney) in a leakproof and sterile container from dead birds, small ruminant or other companion animals. All samples must be shipped chilled overnight.

1- 3 days

Tests run Monday and Thursday.

$30

Cremation Fees 31 lbs. to 150 lbs.

Fee is in addition to necropsies performed on non-food/fiber animals at BADDL. (No charge for cattle, swine, goats, sheep, poultry, captive cervids, alpacas, llamas and rabbits.)

3-7 days

Non-Poultry Only

$100 per animal

Cremation Fees < 30 lbs.

Fee is in addition to necropsies performed on non-food/fiber animals at BADDL. (No charge for cattle, swine, goats, sheep, poultry, captive cervids, alpacas, llamas and rabbits.)

3-7 days

Non-Poultry Only

$50 per animal

Cytology

Air-dried or fixed slides submitted in a plastic or cardboard slide container or leakproof tube for fluid samples. Fluids in a leakproof tube (preferably preserved in EDTA). Unpreserved fluid should be shipped chilled, overnight. Do not ship for Saturday delivery. Culture swabs are unsuitable for cytology.

2 days

$30 per site

Eastern Equine Encephalitis (EEE) Virus qPCR

Mammal: Fresh or frozen brain (preferable brainstem or medulla), spinal cord, or cerebral spinal fluid; shipped chilled overnight. Avian: Fresh or frozen brain, liver, heart, kidney, spleen, or bone marrow, oropharyngeal or cloacal swab, or whole blood; shipped chilled overnight. Mosquitoes - whole body in pools up to 25. Mosquito species: Aedes atlanticus, Aedes sollicitans, Aedes vexans, Coquellitidia perturbans, Culiseta melanura, Culex nigripalpus, Culex quinquefasciatus. Dry mosquito samples must be shipped with dry ice for overnight delivery. Ice packs can be used if samples are shipped within 24 hours of collection for overnight delivery. Mosquito samples in RNAlater can be shipped with ice packs or at room temperature.

1-3 days

Tests run Monday and Thursday.

$30

Eastern Equine Encephalitis (EEE) Virus qPCR

Mammal: Fresh or frozen brain (preferable brainstem or medulla), spinal cord, or cerebral spinal fluid; shipped chilled overnight. Avian: Fresh or frozen brain, liver, heart, kidney, spleen, or bone marrow, oropharyngeal or cloacal swab, or whole blood; shipped chilled overnight. Mosquitoes - whole body in pools up to 25. Mosquito species: Aedes atlanticus, Aedes sollicitans, Aedes vexans, Coquellitidia perturbans, Culiseta melanura, Culex nigripalpus, Culex quinquefasciatus. Dry mosquito samples must be shipped with dry ice for overnight delivery. Ice packs can be used if samples are shipped within 24 hours of collection for overnight delivery. Mosquito samples in RNAlater can be shipped with ice packs or at room temperature.

1- 3 days

Tests run Monday and Thursday.

$30

Fecal Centrifugation Sucrose

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Culture (Aerobic & Salmonella)

Feces in a leakproof container; shipped chilled overnight.

2-5 days (serotyping additional 5-10 days)

$20

Fecal Examination — Direct Smear

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Examination — Zinc Sulfate

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Occult Blood

2 gm of feces in a sealed leakproof container; shipped chilled overnight.

1 day

$10

Fecal Sedimentation

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$8

Fungal Culture

Swabs, fresh tissue or skin scrapings in a sterile container; shipped chilled overnight. Not for environmental samples.

7-28 days

$15

Fungal Identification

Plate or dermatophyte media; shipped chilled overnight. Not for environmental samples.

7-28 days

$10

Gram Stain

Tissues, aspirates, exudates in a sterile leakproof container; shipped chilled overnight. Impression smears on slide within a slide folder.

1 day

$5

Hatchery Air Plate Colony Count

Tryptose agar media exposed to hatchery air environment.

2-3 days

$10

Infectious Laryngotracheitis Virus (ILTV) qPCR

Tracheal or oropharyngeal swabs in 3 ml of viral transport medium (VTM) for pools of up to 5 birds; shipped chilled overnight. Tracheal wash (1-2 mL) or fresh tracheal or lung tissue; shipped chilled overnight. Use synthetic swabs with a plastic handle. Avoid cotton or calcium alginate swabs or swabs with wooden handles.

1-3 days

Tests run Tuesday and Friday.

$25

Large/Complex Dissection

Fresh or fixed large tissues or viscera, double bagged inside a leakproof container; shipped chilled overnight if fresh tissues.

1-2 days

$30

McMaster’s Egg Count

1 gm minimum of fresh feces in a leakproof container; shipped chilled overnight.

1-2 days

$10

Methicillin-resistant Staphylococcus confirmation (mecA qPCR)

Pure Staphylococcus aureus and/or Staphylococcus pseudintermedius isolates; shipped chilled overnight.

1-3 days

Tests run Monday and Thursday.

$30

Methicillin-resistant Staphylococcus confirmation (mecA qPCR)

Pure Staphylococcus aureus and/or Staphylococcus pseudintermedius isolates; shipped chilled overnight.

1-3 days

Tests run Monday and Thursday.

$30

Mycobacterium Culture

Tissue or aspirate, body fluids, or feces in a sterile leakproof container or sealed bag; shipped chilled overnight. Specimens must be submitted as soon as possible, or at most within 24 hours of collection.

56 days

If positive for Mycobacterium growth, sent to FL-DOH or NVSL for identification.

$20

Mycoplasma Culture — Food & Fiber Animal

Fresh tissue from cattle, swine, goats, sheep, poultry, captive cervids, alpacas, llamas, or rabbits in a sterile leakproof container or swabs in Mycoplasma transport medium; shipped chilled overnight and received within 24 hours of collection.

7-10 days

Poultry Only

$10

Mycoplasma Culture — Other Animal

Fresh tissue in a sterile leakproof container or swabs in Mycoplasma transport medium; shipped chilled overnight and received within 24 hours of collection.

7-10 days

Non-Poultry

$15

Mycoplasma gallisepticum Serum Plate Agglutination

1 ml of serum from a chicken or turkey in a serum separator tube (red top/tiger top) or a bullet tube; shipped chilled overnight.

1-3 days

$3

Mycoplasma gallisepticum  (MG)/ Mycoplasma synoviae (MS) qPCR

Tracheal, oropharyngeal, choanal, nasal, or infraorbital sinus swabs in 3 ml of transport medium with no antibiotics for pools of up to 5 birds or respiratory tissue; shipped chilled overnight. Dry swab from individual bird. Moist swabs are preferred; it its recommended to add 1-2 drops of sterile saline to the swab and place in a leakproof and sterile container; shipped chilled overnight. Use synthetic swabs with a plastic handle. Avoid cotton or calcium alginate swabs or swabs with wooden handles.

1-3 days

Tests run Tuesday and Friday.

$25

Mycoplasma synoviae ELISA

1 ml of serum from a chicken in a serum separator tube (red top/tiger top) or other sterile tube; shipped chilled overnight.

3-5 days

Tests run on Thursday for samples received prior to 12:00 PM.

$3

Mycoplasma synoviae Serum Plate Agglutination

1 ml of serum from a chicken or turkey in a serum separator tube (red top/tiger top) or a bullet tube; shipped chilled overnight.

1-3 days

$3

Necropsy Exam — Forensics, Research, and Insurance

Fresh carcass (preferred not frozen) double bagged inside a leakproof container or cooler; shipped chilled overnight.

20 days depending on ancillary testing

$250 per animal plus cremation fee if non-poultry

Necropsy Exam — Non-Poultry Avian

Fresh carcass of a non-poultry avian (preferred not frozen) double bagged inside a leakproof container or cooler; shipped chilled overnight.

20 days depending on ancillary testing

$50 per animal plus cremation fee

Necropsy Exam — Non-Poultry Avian

Fresh carcass of a non-poultry avian (preferred not frozen) double bagged inside a leakproof container or cooler; shipped chilled overnight.

20 days depending on ancillary testing

$50 per animal plus cremation fee

Necropsy Exam — Poultry

Fresh carcass of poultry (chickens, turkeys, ostriches, emus, rheas, cassowaries, waterfowl and game birds, except doves and pigeons) (preferred not frozen), double bagged inside a leakproof Styrofoam or plastic cooler; shipped chilled overnight.

20 days depending on ancillary testing

Includes up to 5 birds

$50

Necropsy in a Bottle (Microscopic Examination)

Fixed tissues from a necropsy not performed at the BADDL shipped in a leakproof container.

10 days

$75 per animal

Newcastle Disease Virus PCR (Avian Paramyxovirus) – Matrix or Velogenic

Poultry - Tracheal or oropharyngeal swabs in 3 ml of viral transport medium (VTM) for pools of up to 5 birds or 5.5 ml of VTM for pools of up to 11 birds; shipped chilled overnight. Captive Pet Birds - Cloacal swabs in 3 ml of VTM broth for pools of up to 5 birds by species; shipped chilled overnight. Wild/Free Roaming Waterfowl – 1 Tracheal and 1 Cloacal from the same bird in 3 ml VTM; shipped chilled overnight. Do not pool more than one bird. Poultry environmental swabs in 5.5 ml of VTM broth for pools of up to 5 swabs. Fresh respiratory, enteric, reproductive, or brain tissue (if neurologic) in a leakproof and sterile container; shipped chilled overnight. Swabs must be received within 4 working days of collection. Use synthetic swabs with a plastic handle. Avoid cotton or calcium alginate swabs or swabs with wooden handles.

1-3 days

Tests run Tuesday and Friday.

$30.00 each No Charge for Poultry Surveillance

Parasite Identification

Larvae or other parasite in 70% alcohol in a sealed leakproof container.

5-10 days

$15

Pullorum disease (PD)/Fowl Typhoid Serum Plate Agglutination

1 ml of serum from an avian in a serum separator tube (red top/tiger top) or a bullet tube; shipped chilled overnight.

1-3 days

$3

Salmonella Culture (Grouping and/or Serotyping on Request)

Chick box papers, drag swabs, boot swabs, chick meconium, and fluff, feces, tissues or body fluids in a sterile, leakproof container; shipped chilled overnight.

2-5 days (serotyping additional 5-10 days)

$15

Salmonella Culture — Serogrouping

Chick box papers, drag swabs, boot swabs, chick meconium, and fluff, feces, tissues or body fluids in a sterile, leakproof container; shipped chilled overnight.

1-2 days

$5

Slide/Block Preparation

Fixed tissue submitted in formalin in a leakproof container. Duplicate slides can be made for send-out.

3-4 days

$15 each

Tick Identification

Tick in 70% alcohol in a sealed leakproof container.

5-10 days

Confirmation of exotic or non-endemic ticks may take up to one month at NVSL.

$15

Virus Isolation

Fresh tissues, swabs, scrapings, body fluids, secretions, urine, blood or whole blood from a non-primate animal submitted in a sterile container.

14-28 days

If required, the suspected cell cultures will be sent to an outside lab for ID confirmation.

$30

Virus Isolation  — Enhanced  — Food and Fiber Animals

Fresh tissues, swabs, scrapings, body fluids, secretions, urine, blood or whole blood from a non-primate animal submitted in a sterile container.

14-28 days

Poultry Only If required, the suspected cell cultures will be sent to an outside lab for ID confirmation.

$30

Virus Isolation — Enhanced — Other Animals

Fresh tissues, swabs, scrapings, body fluids, secretions, urine, blood or whole blood from a non-primate animal submitted in a sterile container.

14-28 days

If required, the suspected cell cultures will be sent to an outside lab for ID confirmation.

$60

West Nile Virus qPCR

Mammal: Fresh or frozen brain (preferable brainstem or medulla), spinal cord, or cerebral spinal fluid; shipped chilled overnight. Avian: Fresh or frozen brain, liver, heart, kidney, spleen, or bone marrow, oropharyngeal or cloacal swab, or whole blood; shipped chilled overnight. Mosquitoes - whole body in pools up to 25. Mosquito species: Culex nigripalpus, Culex quinquefasciatus. Dry mosquito samples must be shipped with dry ice for overnight delivery. Ice packs can be used if samples are shipped within 24 hours of collection for overnight delivery. Mosquito samples in RNAlater can be shipped with ice packs or at room temperature.

1-3 days

Test run Monday and Thursday.

$30