Sheep Tests and Fees

Abortion Bacteria Panel

Fresh tissues, placenta, fetal lung, liver, or stomach content, semen, or cervical or vaginal mucus in a sterile leakproof container; shipped chilled within 24 hours of collection.

5-7 days

Aerobic, Listeria, and Campylobacter culture performed

$15

Acid Fast Stain

Tissues, aspirates, exudates, body fluids, feces in a sterile leakproof container; shipped chilled overnight. Impression smears (skin lesions) on slide within a slide folder.

1 day

$7

ACTH (Baseline)

1 ml of plasma from a purple top tube (EDTA) from all species (no primates or avian); shipped frozen overnight from Monday to Thursday. Collect blood and place it in an EDTA blood tube. Centrifuge within 1 hour of collection, remove the plasma and place 1 ml in an additive free plastic tube. Place the tube in a secondary container with water, freeze the sample in the container filled with water. Ship frozen overnight from Monday to Thursday.

1 day

$30

Aerobic Culture

Fresh tissues or body fluids in a sterile leakproof container or syringe; shipped chilled within 24 hours of collection. Swabs in aerobic transport media; shipped chilled overnight within 24 hours of collection.

3-5 days

$10

Anaerobic Culture

Fresh tissues or body fluids in a sterile leakproof container or syringe; shipped chilled overnight within 24 hours of collection. Swabs in anaerobic transport media; shipped chilled overnight within 24 hours of collection.

3-5 days

$12

Antimicrobial Susceptibility Panels

Pure cultures of isolated pathogen or as requested by the submitter.

1-3 days after ID

Up to 3 isolates per specimen.

$10 per isolate

Bacterial Identification by MALDI-TOF

Culture plates for bacterial ID; shipped chilled within 24 hours of collection.

1-2 days

$10

Baermann Test (Lungworm)

10 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$8

Biopsy

Fixed tissues in a leakproof container with a screw-on lid (should be put in a Ziploc bag with paper towels/absorbent material).

5 days

$40.00 first tissue specimen per animal $10.00 each subsequent tissue specimen

Bluetongue cELISA

1 ml of serum from a bovine, caprine, cervid or ovine in a serum separator tube (red top/tiger top) or other sterile tube; shipped chilled overnight.

3-5 days

Tests run on Wednesday for samples received prior to 12:00 PM.

$7

Bluetongue Virus qPCR

Whole blood in EDTA (purple top), spleen, lung, or lymph node from a bovine, caprine, cervid, alpaca, or ovine; shipped chilled overnight.

1-3 days

Tests run Wednesday and Saturday.

$25

Bluetongue Virus/Epizootic Hemorrhagic Disease qPCR

Whole blood in EDTA (purple top), spleen, lung, or lymph node from a bovine, caprine, cervid, alpaca, or ovine; shipped chilled overnight.

1-3 days

Tests run Wednesday and Saturday.

$30

Border Disease Virus qPCR

Serum, whole blood in EDTA (purple top), plasma, milk, lung, or fresh ear notch from an ovine or caprine in a leakproof and sterile container; shipped chilled overnight.

2-4 days

Tests run Wednesday and Saturday.

$25

Bovine Viral Diarrhea (BVD) Virus Neutralization (VN)

1 ml of serum from a bovine, caprine or ovine in a sterile container; shipped chilled overnight.

4-7 days

$10

Bovine Viral Diarrhea (BVD)  Fluorescent Antibody Test (FA)

Fresh intestine or lung from a bovine, caprine or ovine in a leakproof container; shipped chilled overnight.

1-3 days

$10

Brain Removal and Large Complex Fresh Tissue Handling

Head or fresh carcass (preferred not frozen), double bagged inside a leakproof Styrofoam or plastic cooler; shipped chilled overnight. For rabies testing, contact your county Department of Health (DOH) prior to submission for approval.

1 day

$30 per animal

Brucella abortus/suis Serum Test (BAPA, Card, Rivonal, RAP)

1 ml of serum from a bovine, camelid, caprine, cervid, ovine, porcine or exotic in a serum separator tube (red top/tiger top); shipped chilled overnight.

1-3 days

$5

Brucella melitensis Serum Test (Card)

1 ml of serum from a caprine or ovine in a serum separator tube (red top/tiger top); shipped chilled overnight.

1-3 days

$5

Campylobacter Culture

Aborted fetus or fetal lung, liver, stomach content, placenta, semen, cervical or vaginal mucus in Campylobacter transport medium; shipped chilled overnight or delivered as soon as possible. Feces in a leakproof container or fecal swab in Cary-Blair or another suitable transport media; shipped chilled overnight or delivered as soon as possible.

5-7 days

Fresh or fixed large tissues, viscera, or aborted fetuses submitted to the lab for any test may be charged the Large/Complex Dissection fee if additional handling is required prior to performing the requested test.

$12

CBC Food and Fiber Animals (without differential)

2 ml of blood from a bovine, equine, ovine, caprine, llama, alpaca, or porcine in a purple top tube (EDTA); shipped chilled overnight.

1 day

$10

Chemistry — Large Animal Profile

2 ml of serum from a bovine, ovine, caprine, porcine, alpaca, or llama in a red top tube; shipped chilled overnight. Includes: Albumin (ALB), Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), Calcium (Ca), Creatine Kinase (CK), Total Protein (TP), Gamma Glutamyl Transferase (GGT), Globulin (GLOB), Magnesium (Mg), Phosphorus (PHOS), Blood Urea Nitrogen (BUN).

1 day

$20

Chlamydia spp. qPCR

Cloacal, choanal or throat swabs from live birds. 1 gm of feces from birds in a leakproof and sterile container. 1 gm of fresh tissue (liver, spleen, lung or kidney) in a leakproof and sterile container from dead birds, small ruminant or other companion animals. All samples must be shipped chilled overnight.

1- 3 days

Tests run Monday and Thursday.

$30

Cortisol

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours. Separate serum from clot and ship overnight with ice packs.

1 day

$25

Corynebacterium pseudotuberculosis Synergistic Hemolysin Inhibition (SHI) Test — Caseous Lymphadenitis/Pigeon Fever

0.5 ml of serum from a caprine, ovine, or equine in a serum separator tube (red top/tiger top); shipped chilled overnight.

3-5 days

Tests run on Monday, Tuesday and Thursday for samples received prior to 12:00 pm.

$12

Cryptosporidium Fecal Direct Fluorescent Antigen Test (DFA)

2 gm of fresh feces from all animals in a leakproof container; shipped chilled overnight.

1 day

$20

Cytology

Air-dried or fixed slides submitted in a plastic or cardboard slide container or leakproof tube for fluid samples. Fluids in a leakproof tube (preferably preserved in EDTA). Unpreserved fluid should be shipped chilled, overnight. Do not ship for Saturday delivery. Culture swabs are unsuitable for cytology.

2 days

$30 per site

Eastern Equine Encephalitis (EEE) Virus qPCR

Mammal: Fresh or frozen brain (preferable brainstem or medulla), spinal cord, or cerebral spinal fluid; shipped chilled overnight. Avian: Fresh or frozen brain, liver, heart, kidney, spleen, or bone marrow, oropharyngeal or cloacal swab, or whole blood; shipped chilled overnight. Mosquitoes - whole body in pools up to 25. Mosquito species: Aedes atlanticus, Aedes sollicitans, Aedes vexans, Coquellitidia perturbans, Culiseta melanura, Culex nigripalpus, Culex quinquefasciatus. Dry mosquito samples must be shipped with dry ice for overnight delivery. Ice packs can be used if samples are shipped within 24 hours of collection for overnight delivery. Mosquito samples in RNAlater can be shipped with ice packs or at room temperature.

1- 3 days

Tests run Monday and Thursday.

$30

Electrolyte Plus Panel

1 ml of heparinized whole blood (green top) or serum (red top) from a canine, feline, equine, bovine, caprine, ovine; shipped chilled overnight. Included parameters: Chloride, Potassium, Sodium, tC02

1 day

$15

Epizootic Hemorrhagic Disease Virus qPCR

Whole blood in EDTA (purple top), spleen, lung or lymph node from a bovine, caprine, ovine, alpaca, or cervid; shipped chilled overnight.

1-3 days

Tests run Wednesday and Saturday.

$25

Estradiol

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours. Separate serum from clot and ship overnight with ice packs.

1 day

$30

Fecal Centrifugation Sucrose

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Culture (Aerobic & Salmonella)

Feces in a leakproof container; shipped chilled overnight.

2-5 days (serotyping additional 5-10 days)

$20

Fecal Examination  — Zinc Sulfate

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Examination — Direct Smear

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$10

Fecal Occult Blood

2 gm of feces in a sealed leakproof container; shipped chilled overnight.

1 day

$10

Fecal Sedimentation

2 gm of fresh feces in a leakproof container; shipped chilled overnight.

1 day

$8

Fibrinogen

1 ml of blood from a canine or feline in a blue top tube (Sodium citrate); shipped chilled overnight. 1 ml of blood in a purple top tube (EDTA ) from all other species; shipped chilled overnight.

1 day

$15

Foot and Mouth Disease (FMD) Virus qPCR

Vesicular tissue or fluid, crusts, scabs, oral or lesions swabs from a bovine, porcine, caprine, ovine, camelid or cervid (cloven-hoofed zoo animal or wildlife) in a sterile leakproof tube or container; shipped chilled overnight.

1-3 days

This is an exotic disease. Request the FAD number from State Veterinarian before sample shipment if submission is for NAHLN FAD investigation.

$30

Fungal Culture

Swabs, fresh tissue, or skin scrapings in a sterile container; shipped chilled overnight. Not for environmental samples.

7-28 days

$15

Fungal Identification

Plate or dermatophyte media; shipped chilled overnight. Not for environmental samples.

7-28 days

$10

Giardia Fecal Direct Fluorescent Antigen Test (DFA)

2 gm of fresh feces from all animals in a leakproof container; shipped chilled overnight.

1 day

$20

Gram Stain

Tissues, aspirates, exudates in a sterile leakproof container; shipped chilled overnight. Impression smears on slide within a slide folder.

1 day

$5

Influenza A qPCR Matrix (Mammalian)

Respiratory or nasal swabs in 3 ml of viral transport medium (VTM) or fresh lung; shipped chilled overnight. Samples accepted from feline, equine, porcine, canine and pinnipeds. Do not pool swabs. Swabs must be received within 4 working days of collection. Use synthetic swabs with a plastic handle. Avoid cotton or calcium alginate swabs or swabs with wooden handles.

1-3 days

Tests run Tuesday and Friday.

$30

Insulin

1 ml of serum in a red top tube from all species (no primates or avian); shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$25

Johne’s Disease (Mycobacterium avium subspecies  paratuberculosis) qPCR

1 gm of feces from a bovine, caprine, or ovine in a leakproof container; shipped chilled overnight. 1 gm of feces for up to 5 animals each in a separate sterile container; shipped chilled overnight. 1) Large groups of samples can be combined into pools of 5 for a discounted price. This pooling will only be done at BADDL. 2) Submit samples individually.

2-4 days

Tests run Monday and Thursday.

$20.00 Individual $30.00 Pooled

Johne’s Disease ELISA

1 ml of serum from a bovine, caprine, or ovine in a serum separator tube (red top/tiger top) or other sterile tube; shipped chilled overnight. The IDEXX MAP Ab Test kit is a USDA approved Johne's ELISA kit for the detection of antibodies to Mycobacterium avium ssp. paratuberculosis in bovine serum or plasma. This test is to be used as an initial screening method for the testing of small ruminants.

3-5 days

Tests run on Tuesday for samples received prior to 12:00 PM. Tests run on Friday for samples received by 5:00 PM the day prior.

$6

Large/Complex Dissection

Fresh or fixed large tissues or viscera, double bagged inside a leakproof container; shipped chilled overnight if fresh tissues.

1-2 days

$30

Leptospira spp. qPCR

Urine: minimum of 5 mL for small animals or 10 mL for large animals in leakproof and sterile container; shipped chilled overnight. Tissue: kidney, liver, placenta in leakproof and sterile container; shipped chilled overnight. Whole blood in EDTA (purple top) tube; shipped chilled overnight. Animal species: multiple species

2-4 days

Tests run on Tuesday and Friday.

$30

Leptospirosis IHC

Kidney in formalin in a leakproof container.

10 days

$25

Leptospirosis Microscopic Agglutination Test 5 (Tests for 5 serovars: L. Pomona; L. ictero; L. canicola; L. grippo and L. hardjo)

1 ml of serum in a serum separator tube (red top/tiger top); shipped chilled overnight.

3-5 days

Tests run on Wednesday. Samples received prior to 10:30 AM on Wednesday will be tested same day.

$15

Mastitis Screening Bacterial Culture

Fresh tissues, abscess, or 3 ml of milk or colostrum in a sterile leakproof container or syringe; shipped chilled to arrive within 24 hours of collection. Includes aerobic and Mycoplasma culture.

3-10 days

$15

McMaster’s Egg Count

1 gm minimum of fresh feces in a leakproof container; shipped chilled overnight.

1-2 days

$10

Methicillin-resistant Staphylococcus confirmation (mecA qPCR)

Pure Staphylococcus aureus and/or Staphylococcus pseudintermedius isolates; shipped chilled overnight.

1-3 days

Tests run Monday and Thursday.

$30

Mycobacterium Culture

Tissue or aspirate, body fluids, or feces in a sterile leakproof container or sealed bag; shipped chilled overnight. Specimens must be submitted as soon as possible, or at most within 24 hours of collection.

56 days

If positive for Mycobacterium growth, sent to FL-DOH or NVSL for identification.

$20

Mycoplasma Culture  — Food & Fiber Animal

Fresh tissue from cattle, swine, goats, sheep, poultry, captive cervids, alpacas, llamas or rabbits in a sterile leakproof container or swabs in Mycoplasma transport medium; shipped chilled overnight and received within 24 hours of collection.

7-10 days

$10

Necropsy Exam  — Food and Fiber Animal

Fresh carcass of cattle, swine, goats, sheep, poultry, rabbits, captive cervids, llamas or alpacas (preferred not frozen), double bagged inside a leakproof container or cooler; shipped chilled overnight.

20 days depending on ancillary testing

$100 per animal

Necropsy Exam  — Forensics, Research, and Insurance

Fresh carcass (preferred not frozen) double bagged inside a leakproof container or cooler; shipped chilled overnight.

20 days depending on ancillary testing

$250 per animal

Necropsy in a Bottle (Microscopic Examination)

Fixed tissues from a necropsy not performed at the BADDL shipped in a leakproof container.

10 days

$75 per animal

Parasite Identification

Larvae or other parasite in 70% alcohol in a sealed leakproof container.

5-10 days

$15

Phenobarbital

1 ml of serum in a red top tube from all species (no primates or avian); shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$30

Pregnancy Test

1 ml of serum from cattle, caprine, or ovine in a serum separator tube (red top/tiger top) or other sterile tube or 1 ml of plasma from cattle, water buffalo, or bison in an EDTA (purple top) tube; shipped chilled overnight.

2-3 days

Pregnancy may be detected in cattle and goat samples 28 days post-breeding, 35 days in sheep, and 30 days in bison. This test can be used ≥ 60 days post calving in cattle and bison. For sheep and goat no post kidding claim is given.

1-19 samples: $4.00/test >20 samples: $3.00/test

Progesterone

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a non-additive clot tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples. Usually within the next 4 hours to avoid loss of progesterone.

1 day

$20

Rabies (Non-Exposure)

Head or carcass (not frozen), double bagged inside a leakproof container; shipped chilled overnight. Requires the examination of a complete cross-section of the brain stem and cerebellum (including vermis, right & left lobes). If the cerebellum is not available, a diagnosis can be made by the examination of the brain stem and hippocampi (right and left).

1-3 days

$40

Reticulocyte Count

2 ml of blood in a purple top tube (EDTA); shipped chilled overnight.

1 day

$8

RPLN/Obex Collection

Head or fresh carcass (preferred not frozen), double bagged inside a leakproof container or cooler; shipped chilled overnight.

1-2 days

$30 per animal

Salmonella Culture (Grouping and/or Serotyping on Request)

Chick box papers, drag swabs, boot swabs, chick meconium, and fluff, feces, tissues, or body fluids in a sterile, leakproof container; shipped chilled overnight.

2-5 days (serotyping additional 5-10 days)

$15

Salmonella Culture - Serogrouping

Chick box papers, drag swabs, boot swabs, chick meconium, and fluff, feces, tissues, or body fluids in a sterile, leakproof container; shipped chilled overnight.

1-2 days

$5

Scrapie IHC

Tonsils, retropharyngeal lymph nodes and obex from a caprine or ovine in formalin in a leakproof container.

10 days

$25

Slide/Block Preparation

Fixed tissue submitted in formalin in a leakproof container. Duplicate slides can be made for send-out.

3-4 days

$15 each

Small Ruminant Lentivirus cELISA (CAE/OPP)

1 ml of serum from a caprine or ovine in a serum separator tube (red top/tiger top); shipped chilled overnight.

2-3 days

Tests run on Tuesday and Thursday for samples received prior to 12:00 PM.

$7

Spinal Cord Removal

Fresh carcass (preferred not frozen), double bagged inside a leakproof Styrofoam or plastic cooler; shipped chilled overnight.

20-25 days

$50 per animal

Testosterone

1 ml of serum in a red top tube from all species (no primates or avian); shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$20

Thyroid Panels – TSH+TP1 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$30 (includes TSH + 1 choice in panel)

Thyroid Panels – TSH+TP4 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$55 (includes TSH + 4 choices in panel)

Thyroid Panels – TSH+TP4 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$55 (includes TSH + 4 choices in panel)

Thyroid Panels  — TSH+TP3 (TT3, TT4, FT3, FT4, Tg)

1 ml of serum from all species (no primates or avian) in red top tube; shipped chilled overnight from Monday to Thursday. Collect blood in a red top tube to allow clot formation. Centrifuging serum samples before a complete clot forms may result in the presence of fibrin. Ensure complete clot formation prior to centrifugation of samples, usually within the next 4 hours.

1 day

$45 (includes TSH + 3 choices in panel)

Tick Identification

Tick in 70% alcohol in a sealed leakproof container.

5-10 days

Confirmation of exotic or non-endemic ticks may take up to one month at NVSL.

$15

Urine Analysis (Cytology & Fluid Chemistry Analysis)

10-15 ml urine in a sealed leakproof tube; shipped chilled overnight.

1 day

$35

Vesicular Stomatitis (VS) Virus Neutralization (Indiana Strain)

1 ml of serum from a bovine, equine, porcine, caprine or ovine in a sterile container; shipped chilled overnight.

4-7 days

Tests run Monday, Wednesday and Friday for samples received the day prior. Suspect samples forwarded to NVSL for additional analysis and confirmation. * Unless specified on the submission form, samples submitted will have both Indiana and New Jersey strains VN performed.

$12 per strain

Vesicular Stomatitis (VS) Virus Neutralization (New Jersey Strain)

1 ml of serum from a bovine, equine, porcine, caprine or ovine in a sterile container; shipped chilled overnight.

4-7 days

Tests run Monday, Wednesday and Friday for samples received the day prior. Suspect samples forwarded to NVSL for additional analysis and confirmation. * Unless specified on the submission form, samples submitted will have both Indiana and New Jersey strains VN performed.

$12 per strain

Virus Isolation

Fresh tissues, swabs, scrapings, body fluids, secretions, urine, blood or whole blood from a non-primate animal submitted in a sterile container.

14-28 days

If required, the suspected cell cultures will be sent to an outside lab for ID confirmation.

$30

Virus Isolation

Fresh tissues, swabs, scrapings, body fluids, secretions, urine, blood or whole blood from a non-primate animal submitted in a sterile container.

14-28 days

If required, the suspected cell cultures will be sent to an outside lab for ID confirmation.

$30